Application

Immunofluorescence

ELISA (for detection only)

Does not work for Western Blot

Does not work for Immunohistochemistry

Optimal working dilutions must be determined by end user.

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

Detect Survivin using this Anti-Survivin Antibody, clone 8E2 validated for use in ELISA & IF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Survivin, an inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin′s anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It indicates that survivin may counteract a default induction of apoptosis in G2/M phase. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

Immunogen

Full length recombinant human survivin

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB3509

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin, by Protein G. Liquid in 10 mM PBS, pH 7,4 containing 0.2% BSA and 0.09% sodium azide.

Format: Purified

Specificity

MAB4617 detects human survivin (16.5 kDa).

Storage and Stability

Maintain at 2-8°C for up to 12 months.